Changes in isoform variants and relative abundancies are an important clue in deciphering how genomic variants drive the phenotypic differences between health and disease.
Only long-read sequencing can provide unbiased, direct detection of all the isoforms present in the samples.
Iso-sequencing analysis allows us to generate full length cDNA sequences without assembly.
It characterises transcript isoforms within targeted genes or across an entire transcriptome.
Bioinformatics Analysis
- Data quality control
- Annotation of gene Function, CDS prediction and SSR detection
- Expression and differential expression
- GO and KEGG
(More analysis upon request)
Advantages
- Discover new genes, transcripts, and alternative splicing events
- Improve genome annotation to identify gene structure, regulatory elements, and coding regions
- Increase the accuracy of RNA-sequencing quantification with isoform-level resolution
Sequencing Platforms
PacBio Sequel
Analysis Workflow
For more information, feel free to contact us via NGS@biobasic-asia.com or +65 6928 9042.