Glutathione Beads 4FF is an affinity chromatography medium for the fast and efficient one-step purification of glutathione S-transferase (GST)-tagged proteins, other GSTs and glutathione-binding proteins. GST-tagged proteins produced through the pGEX series of expression vectors can be purified from the pre-treated bacterial lysate, while using Glutathione Beads 4FF.
GST-tagged proteins binds to the glutathione ligand with high affinity. The coupling of the glutathione ligand to highly cross-linked 4% agarose beads optimized the binding capacity for GST-tagged proteins and other glutathione-binding proteins. Elution of the bound GST-tagged proteins are operated under mild and non-denaturing conditions, with Elution Buffer containing reduced glutathione, to preserve the protein antigenicity and functions. The high flow properties of Glutathione Beads 4FF make it excellent for scaled up purification of GST-tagged protein.