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Glutathione Beads 4FF


Glutathione Beads 4FF is an affinity chromatography medium for the fast and efficient one-step purification of glutathione S-transferase (GST)-tagged proteins, other GSTs and glutathione-binding proteins.

Catalog Number Product Size (mL) Particle Size (μm)
SA010010 10 45-165
SA010050 50 45-165
SA010100 100 45-165

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Glutathione Beads 4FF is an affinity chromatography medium for the fast and efficient one-step purification of glutathione S-transferase (GST)-tagged proteins, other GSTs and glutathione-binding proteins. GST-tagged proteins produced through the pGEX series of expression vectors can be purified from the pre-treated bacterial lysate, while using Glutathione Beads 4FF.

GST-tagged proteins binds to the glutathione ligand with high affinity. The coupling of the glutathione ligand to highly cross-linked 4% agarose beads optimized the binding capacity for GST-tagged proteins and other glutathione-binding proteins. Elution of the bound GST-tagged proteins are operated under mild and non-denaturing conditions, with Elution Buffer containing reduced glutathione, to preserve the protein antigenicity and functions. The high flow properties of Glutathione Beads 4FF make it excellent for scaled up purification of GST-tagged protein.

Specifications

Chromatography Technique Tagged protein purification
Matrix Highly cross-linked 4% agarose
Ligand Glutathione (coupled via a 12-atom linker arm)
Ligand Concentration 120-320 μmol glutathione/ml medium
Binding Capacity >10 mg GST-fusion proteins/ml medium
Particle Size 45μm-165μm
Maximum Pressure 3 bar (0.3 MPa, 43 psi)
Maximum Flow Rate 450 cm/h
pH Stability pH 3-12
Storage Conditions 2°C-8°C; 1xPBS containing20% ethanol (Do not freeze)

Documents

Product Manual