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Glutathione Beads


Glutathione Beads is an affinity chromatography medium designed for the high performance purification of recombinant glutathione S-transferase (GST) fusion proteins and glutathione-binding proteins expressed in E.coli and mammalian cells.

Catalog Number Product Size (mL) Particle Size (μm)
SA008010 10 45-165
SA008025 25 45-165
SA008050 50 45-165
SA008100 100 45-165

To get product quotation, you may email sg@biobasic.com.

Glutathione Beads is an affinity chromatography medium designed for the high performance purification of recombinant glutathione S-transferase (GST) fusion proteins and glutathione-binding proteins expressed in E.coli and mammalian cells.

GST-tagged proteins binds to the glutathione ligand with high affinity. The coupling of glutathione ligand to 4% agarose beads optimized the binding capacity for GST-tagged proteins and other glutathione-binding proteins. Elution of the GST-tagged proteins are performed under mild and non-denaturing conditions, with Elution Buffer containing reduced glutathione, to preserve the protein antigenicity and functions.

Specifications

Chromatography Technique Tagged protein purification
Matrix 4% agarose beads
Ligand Glutathione
Ligand Concentration 300 μmol glutathione
Binding Capacity* >20 mg GST/ml medium
Particle Size 45μm-165μm
Maximum Pressure 1 bar (0.1 MPa)
Recommended Flow Rate <75 cm/h
pH Stability pH 3-12
Storage Conditions 2°C-8°C; 20% ethanol

Documents

Product Manual