Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium designed for the purification of histidine-tagged proteins in eukaryotic cell culture supernatant (i.e. mammalian and insect). The Ni Smart Beads 6FF is pre-charged with nickel ions through a proprietary, chemically stable linker technology. The strong binding between nickel ions and His-tagged proteins provides excellent resistance against EDTA and reducing agents (i.e. DTT and β-mercaptoethanol). Unlike conventional IMAC mediums, direct loading of large amounts of untreated culture samples is possible in Ni Smart Beads 6FF, without requiring a preceding step to eliminate EDTA and other nickel-chelating agents.
Affinity Chromatography, Protein Purification Beads, Tagged Proteins
Ni Smart Beads 6FF
Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium designed for the purification of histidine-tagged proteins in eukaryotic cell culture supernatant (i.e. mammalian and insect).
| Catalog Number | Product Size (mL) | Particle Size (μm) |
| SA036010 | 10 | 45-165 |
| SA036050 | 50 | 45-165 |
| SA036100 | 100 | 45-165 |
To get product quotation, you may email sg@biobasic.com.
Specifications
| Matrix | Highly cross-linked 6% agarose |
| Binding Capacity | >10 mg 6xHis-tagged protein/mL medium |
| Particle Size | 45μm-165μm |
| Maximum Pressure | 3 bar (0.3 MPa) |
| Storage Conditions | 2°C-8°C; 1xPBS containing 20% ethanol |
Documents
Product Manual
