rProtein A Beads 4FF is an affinity chromatography medium designed for the one-step purification of immunoglobulins from biological fluids and cell culture media. Coupling of recombinant protein A ligand to highly cross-linked 4% agarose beads optimized the binding capacity of rProtein A Beads 4FF to immunoglobulins, especially IgG-class antibodies. The dynamic binding capacity of rProtein A Beads 4FF is dependent on various factors, such as target antibody and flow rate.
Protein A is a cell wall component present in several strains of Staphylococcus aureus. IgG-class antibodies bind to the IgG binding domains of the immobilized recombinant protein A ligand through interactions with the Fc regions of mammalian IgG, with high specificity and affinity.