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rProtein A Beads 4FF


rProtein A Beads 4FF is an affinity chromatography medium designed for the one-step purification of immunoglobulins from biological fluids and cell culture media.

Catalog Number Product Size (mL) Particle Size (μm)
11-0203-005 5 45-165
11-0203-025 25 45-165
11-0203-200 200 45-165

To get product quotation, you may email sg@biobasic.com.

rProtein A Beads 4FF is an affinity chromatography medium designed for the one-step purification of immunoglobulins from biological fluids and cell culture media. Coupling of recombinant protein A ligand to highly cross-linked 4% agarose beads optimized the binding capacity of rProtein A Beads 4FF to immunoglobulins, especially IgG-class antibodies. The dynamic binding capacity of rProtein A Beads 4FF is dependent on various factors, such as target antibody and flow rate.

Protein A is a cell wall component present in several strains of Staphylococcus aureus. IgG-class antibodies bind to the IgG binding domains of the immobilized recombinant protein A ligand through interactions with the Fc regions of mammalian IgG, with high specificity and affinity.

Specifications

Chromatography Technique Antibody affinity chromatography
Matrix Highly cross-linked 4% agarose beads (supplied as 50% slurry)
Ligand Recombinant protein A
Particle Size 45 μm-165 μm
Binding Capacity >40 mg human IgG/ml medium
Temperature Stability 2°C-40°C
Maximum Pressure 3 bar (0.3 MPa)
pH Stability Working range: pH 2-9
Cleaning-in-Place: pH 2-11
Chemical Stability Stable in commonly used aqueous buffers:

  • 10 mM HCl (pH 2),
  • 1 mM NaOH (pH 11),
  • 0.1 M sodium citrate/HCl (pH 3),
  • 6 M guanidine-HCl,
  • 20% ethanol
Flow Velocity 30-300 cm/h

Documents

Product Manual