rProtein A Beads is an affinity chromatography medium designed for the easy one-step purification of immunoglobulins from biological fluids and cell culture media. Coupling of recombinant protein A ligand to 4% agarose beads optimized the binding capacity of rProtein A Beads to immunoglobulins, especially IgG-class antibodies. The dynamic binding capacity of rProtein A Beads is dependent on various factors, such as target antibody and flow rate.
Protein A is a bacterial cell wall protein in Staphylococcus aureus. IgG-class antibodies bind to the IgG binding domains of the immobilized recombinant protein A ligand through interactions with the Fc regions of mammalian IgG, with high specificity and affinity. The Fab region in recombinant protein A is involved in the binding of antigens, making rProtein A Beads excellent for isolation of immune complexes.