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rProtein A Beads


rProtein A Beads is an affinity chromatography medium designed for the easy one-step purification of immunoglobulins from biological fluids and cell culture media.

Catalog Number Product Size (mL) Particle Size (μm)
SA012005 5 45-165
SA012025 25 45-165
SA012200 200 45-165

To get product quotation, you may email sg@biobasic.com.

rProtein A Beads is an affinity chromatography medium designed for the easy one-step purification of immunoglobulins from biological fluids and cell culture media. Coupling of recombinant protein A ligand to 4% agarose beads optimized the binding capacity of rProtein A Beads to immunoglobulins, especially IgG-class antibodies. The dynamic binding capacity of rProtein A Beads is dependent on various factors, such as target antibody and flow rate.

Protein A is a bacterial cell wall protein in Staphylococcus aureus. IgG-class antibodies bind to the IgG binding domains of the immobilized recombinant protein A ligand through interactions with the Fc regions of mammalian IgG, with high specificity and affinity. The Fab region in recombinant protein A is involved in the binding of antigens, making rProtein A Beads excellent for isolation of immune complexes.

Specifications

Chromatography Technique Antibody affinity chromatography
Matrix 4% agarose beads
Ligand Recombinant protein A
Particle Size 45 μm-165 μm
Binding Capacity >40 mg human IgG/ml medium
Temperature Stability 2°C-40°C
Maximum Pressure 1 bar (0.1 MPa)
pH Stability Short Term: pH 3-9
Long Term: pH 2-10
Chemical Stability Stable in commonly used aqueous buffers
(i.e. 6 M guanidine-hydrochloride and 8 M urea)
Storage Conditions 2°C-8°C; 20% ethanol (Do not freeze)

Documents

Product Manual