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rProtein G Beads


rProtein A Beads is an affinity chromatography medium designed for the easy one-step purification of immunoglobulins from biological fluids and cell culture media.

Catalog Number Product Size (mL) Particle Size (μm)
SA016005 5 45-165
SA016025 25 45-165
SA016100 100 45-165

To get product quotation, you may email sg@biobasic.com.

rProtein G Beads is an affinity chromatography medium designed for the one-step purification of immunoglobulins from biological fluids and cell culture media. The coupling of recombinant protein G ligand to 4% agarose beads in rProtein G Beads optimizes the binding capacity to immunoglobulins. With the chemical stability and high batch-to-batch reproducibility of the medium, rProtein G Beads is excellent for isolating immune complexes.

Protein G is a bacterial cell wall component isolated from group G Steptococci. Likewise to Protein A, Protein G binds to the Fc regions of mammalian IgG. The non-specific albumin and cell surface binding sites in recombinant protein G have been eliminated, while maintaining the IgG binding sites. The binding characteristics of protein G and protein A are attributed to their significantly different amino acid compositions. In comparison with Protein A, Protein G can be used for the purification of a wider spectrum of mammalian (i.e. human, mouse and rat) monoclonal and polyclonal IgG-class antibodies at high binding affinity, but not with other classes of immunoglobulins (i.e. IgM, IgD and IgA).

Specifications

Chromatography Technique Antibody affinity chromatography
Matrix 4% agarose beads
Ligand Recombinant protein G
Particle Size 45 μm-165 μm
Binding Capacity >30 mg human IgG/ml medium
Chemical Stability IgG binding capacity and recovery is maintained after storage for:

7 days at 37°C in:
1 M acetic acid, pH 2.0; 20 mM sodium phosphate, 1% SDS, pH 7.0; 6 M Guandine-HCl, pH 7.0; 70% ethanol.

2 hours at room temperature in:
0.1 M HCl, pH 1.0; 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11.

Maximum Pressure 1 bar (0.1 MPa)
pH Stability Long Term pH 3-9
Short Term: pH 2-10
Storage Conditions 2°C-8°C; 1xPBS containing 20% ethanol (Do not freeze)

Documents

Product Manual