Covid-19 Related

COVID-19 Primers and Probes

To help speed up the generation of a vaccine against the virus, Bio Basic Asia Pacific is now providing ready-to-use primers and probes for COVID-19 (Coronavirus). These ready-to-use primers and probes will be made available to ship asap* with quick re-supply whenever necessary. We aim to prioritize all Coronavirus (COVID-19) related projects for as long as it is needed.

*: Probes may have an additional turnaround of 3-5 days.

Real time RT-PCR Primer list obtained from the United States Centers for Disease Control and Prevention (CDC) – last updated March 6, 2020.
Primers, Probes and Protocol recommended by the World Health Organization (WHO) also available.

Name Sequence Length Final Yield Purification Modification Product information
2019-nCoV_N1-F 5′-GAC CCC AAA ATC AGC GAA AT-3′ 20 2 OD HPLC Click here
2019-nCoV_N1-R 5′-TCT GGT TAC TGC CAG TTG AAT CTG-3′ 24 2 OD HPLC Click here
2019-nCoV_N1-P 5′-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3′ 24 2 OD HPLC 5’6-FAM, 3’BHQ1 Click here
2019-nCoV_N2-F 5′-TTA CAA ACA TTG GCC GCA AA-3′ 20 2 OD HPLC Click here
2019-nCoV_N2-R 5′-GCG CGA CAT TCC GAA GAA-3′ 18 2 OD HPLC Click here
2019-nCoV_N2-P 5′-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3′ 23 2 OD HPLC 5’6-FAM, 3’BHQ1 Click here
2019-nCoV_N3-F 5′-GGG AGC CTT GAA TAC ACC AAA A-3′ 22 2 OD HPLC Click here
2019-nCoV_N3-R 5′-TGT AGC ACG ATT GCA GCA TTG-3′ 21 2 OD HPLC Click here
2019-nCoV_N3-P 5′-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3′ 24 2 OD HPLC 5’6-FAM, 3’BHQ1 Click here
RNAse P (RP-F) 5′-AGA TTT GGA CCT GCG AGC G-3′ 19 2 OD HPLC Click here
RNAse P (RP-R) 5′-GAG CGG CTG TCT CCA CAA GT-3′ 20 2 OD HPLC Click here
RNAse P (RP-P) 5′-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ-1-3′ 23 2 OD HPLC 5’6-FAM, 3’BHQ1 Click here

For technical support assistance or other enquiries, please contact our local sales team:

E-mail: order.sg@biobasic.com / sg@biobasic.com
Tel: +65 8284 0161

COVID-19 Viral RNA Extraction Kit

These Viral RNA extraction kits are especially designed for COVID-19 RNA Extraction. The kit simplifies the isolation of viral RNA from cell‐free body fluids using a fast spin‐column format. No phenol/chloroform extraction is required. Viral RNA binds specifically, while contaminants are removed in the flow‐through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in RNase‐free water. Purified RNA is ready to use in RT‐PCR, Northern blotting, or other downstream applications.

Kits are available in spin-column or magnetic bead formats.

Cat # Description Size Product Information
VT9200 COVID-19 Viral RNA Extraction Kit (EZ-10 Spin Column) 50 Preps Click here
VT9100 COVID-19 Viral RNA Extraction Kit (MagicMag Magnetic Beads) 100 Preps Click here
MAD-01 96‐well magnetic separation device (for Magnetic Beads) 1 Click here
MAD-02 Micro‐centrifuge magnetic separation device (for Magnetic Beads) 1 Click here

For technical support assistance or other enquiries, please contact our local sales team:

E-mail: order.sg@biobasic.com / sg@biobasic.com
Tel: +65 8284 0161

COVID-19 Related Proteins

In collaboration with Cohesion Biosciences (http://www.cohesionbio.com/), Bio Basic Asia Pacific has made COVID-19 Proteins available in order to support research needs. We aim to prioritize all COVID-19 related projects for as long as it is needed.

Cat No. Product Name Source Expression Region Tag Accession/

Gene ID

CRP1003 Recombinant Human ACE2 Protein Human cells Gln18-Ser740 6His tag C-terminus 340273
CRP3093 Recombinant COVID-19 3C-like Proteinase E. coli Ser1-Gln306 6His tag C-terminus YP_009725301.1
CRP3094 Recombinant COVID-19 S Protein RBD Human cells Asn331-Val524 mFc tag C-terminus QHD43416.1
CRP3095 Recombinant COVID-19 S Protein RBD Human cells Asn331-Val524 6His tag C-terminus QHD43416.1
CRP3096 Recombinant COVID-19 S Protein RBD-SD1 Human cells Arg319-Pro589 mFc tag C-terminus QHD43416.1
CRP3097 Recombinant COVID-19 S Protein RBD-SD1 Human cells Arg319-Ser591 6His tag C-terminus QHD43416.1
CRP3098 Recombinant COVID-19 S1 Protein Human cells Gin18-Ser740 mFc tag C-terminus QHD43416.1
CRP3099 Recombinant COVID-19 S1 Protein Human cells Gin18-Ser740 Fc tag C-terminus QHD43416.1
CRP3100 Recombinant COVID-19 S1 Protein Human cells Gin18-Ser740 QHD43416.1
CRP3101 Recombinant COVID-19 S1 Protein Human cells Val16-Arg685 6His tag C-terminus QHD43416.1
CRP3102 Recombinant COVID-19 S1 Protein CTD Human cells 8His&Flag tag N-terminus QHD43416.1
CRP3103 Recombinant COVID-19 S

Protein NTD

Human cells 6His tag C-terminus QHD43416.1
CRP3104 Recombinant COVID-19

Nucleocapsid Protein

E. coli Met1-Ala419 6His tag C-terminus QHD43423.2
CRP3105 Recombinant COVID-19

Nucleocapsid Protein NTD

E. coli 6His tag C-terminus QHD43423.2
CRP3106 Recombinant COVID-19

Nucleocapsid Protein CTD

E. coli 6His tag C-terminus QHD43423.2
CRP3107 Recombinant COVID-19

Envelope Protein

E. coli Met1-Val75 BBP&6His tag N-terminus QHD43418.1
CRP3708 Recombinant COVID-19 Spike

Protein

Human cells Gin18-Ser740 6His tag C-terminus QHD43418.1

References

  1. F Wu, et al. A new coronavirus associated with human respiratory disease in China.
    Nature. 579, 265–269 (2020).
  2. M Hoffmann, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 181, 1–10 (2020).
  3. N Dong, et al. Genomic and protein structure modelling analysis depicts the origin and infectivity of 2019-nCoV, a new coronavirus which caused a pneumonia outbreak in Wuhan, China. bioRxiv (2020).

For technical support assistance or other enquiries, please contact our local sales team:

E-mail: order.sg@biobasic.com / sg@biobasic.com
Tel: +65 8284 0161